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Following centrifugation, the supernatant was incubated with 50 l Strep-Tactin Sepharose resin, MBP resin, or GST beads (Beyotime, P2253) at 4 C for 1 h

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It is classified as a "conditionally essential" amino acid

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FeiFei W, HongHai X, YongRong Y, PingXiang W, JianHua W, XiaoHui Z, JiaoYing L, JingBo S, Kun Z, XiaoLi R, Lu Q, XiaoLiang L, ZhiQiang C, Na T, WenTing L, YanQing D, Li L (2019) FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression